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ugtP [2017-10-11 11:43:31]
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ugtP [2017-10-11 11:43:31]

UDP-glucose diacylglycerol glucosyltransferase, growth-rate dependent inhibitor of cell division
locus
BSU21920
pI
8.40
mw
43.40 kDa
protein length
382 aa Sequence Blast
gene length
1146 bp Sequence Blast
function
synthesis of glucolipids and anchoring of lipoteichoic acid, inhibition of FtsZ assembly
product
UDP-glucose diacylglycerol glucosyltransferase
essential
no
ec
2.4.1.315
synonyms
ypfP
Outlinks
KEGGBsubCycSubtiListUniProtExpression Browser

Genomic Context

      

categories

  • [category|SW 1|Cellular processes] → [category|SW 1.1|Cell envelope and cell division] → [category|SW 1.1.2|Cell shape]
  • [category|SW 1|Cellular processes] → [category|SW 1.1|Cell envelope and cell division] → [category|SW 1.1.8|Cell division] → [category|SW 1.1.8.2|Other genes]
  • [category|SW 2|Metabolism] → [category|SW 2.4|Lipid metabolism] → [category|SW 2.4.3|Lipid metabolism/ other]
  • [category|SW 4|Lifestyles] → [category|SW 4.3|Coping with stress] → [category|SW 4.3.2|Cell envelope stress proteins (controlled by SigM, V, W, X, Y)]
  • [category|SW 6|Groups of genes] → [category|SW 6.2|Membrane proteins]

    • Gene

    Coordinates
    2,306,514 → 2,307,662

    Phenotypes of a mutant

  • cells are bent and distended due to the lack of glucolipids [Pubmed|22362028,27684739]
  • increased expression of the [protein|081DF3EE9FA56209D648C7677188C61CE3AA8E41|SigM], [protein|D04629D798E458AD538809BB2B150B7FA81D2C7E|SigV], and [protein|E77364F274A520FCCA9F5C9504E317B047BA29E6|SigX] regulons [Pubmed|22362028]
  • altered [SW|localization] of [protein|A4C8719E06F774A6EB4D79757CC79CF89E453A54|MreB] (irregular clusters instead of helical dots) [Pubmed|22362028]
  • the inactivation of ''[gene|7A606B8E952AE8CA4F9A62008BA4B156725BB5B5|ugtP]'' suppresses the poor and filamentous growth of the ''[gene|1986DB3078FBA16D6ACCD70EEAC95C46564D0958|whiA] [gene|EAD44AA7D72510B70308FD0C9F159DBEDAEBBB74|zapA]'' double mutant [Pubmed|24097947]
  • increased conjugation of ICEBs1 [Pubmed|25069588,26833415]

    • The protein

    Catalyzed reaction/ biological activity

  • UDP-glucose + 1,2-diacylglycerol = UDP + 1,2-diacyl-3-(O-beta-D-glucopyranosyl)-sn-glycerol (according to Swiss-Prot)
  • the interaction with [protein|41872E2EF00C79918DD077F2EF78F37E24FEB110|FtsZ] results in inhibition of [SW|cell division] and an increase of cell size [Pubmed|22931116]

    • Effectors of protein activity

  • oligomerization of [protein|7A606B8E952AE8CA4F9A62008BA4B156725BB5B5|UgtP] is inhibited by UDP-Glc and by interaction with [protein|41872E2EF00C79918DD077F2EF78F37E24FEB110|FtsZ] [Pubmed|22931116]

    • [SW|Localization]

  • membrane-bound protein, self-assembles into tightly wound spirals ''in vitro'' [Pubmed|17662947]
  • under nutrient rich conditions (increased concentration of UDP-Glc): throughout the cell, concentrated at the cell poles and/or the cytokinetic ring, interaction with [protein|41872E2EF00C79918DD077F2EF78F37E24FEB110|FtsZ] [Pubmed|22931116]
  • under nutrient poor conditions: forms punctate foci (oligomers), no interaction with [protein|41872E2EF00C79918DD077F2EF78F37E24FEB110|FtsZ] [Pubmed|22931116]

    • Expression and Regulation

    Operons

    genes
    [gene|7A606B8E952AE8CA4F9A62008BA4B156725BB5B5|ugtP]
    description
    [Pubmed|22383849]

    sigma factors

  • [protein|081DF3EE9FA56209D648C7677188C61CE3AA8E41|SigM]: sigma factor, [Pubmed|18179421], in [regulon|081DF3EE9FA56209D648C7677188C61CE3AA8E41|SigM regulon]
    • view in new tab

    genes
    [gene|1217330ECD588E24E42FF9FED2A097CEF32785F3|metA]-[gene|7A606B8E952AE8CA4F9A62008BA4B156725BB5B5|ugtP]
    description
    [Pubmed|22383849]

    sigma factors

  • [protein|081DF3EE9FA56209D648C7677188C61CE3AA8E41|SigM]: sigma factor, [Pubmed|18179421], in [regulon|081DF3EE9FA56209D648C7677188C61CE3AA8E41|SigM regulon]
    • view in new tab

    Biological materials

    Mutant

  • MGNA-A886 (ypfP::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/886 NBRP B. subtilis, Japan]
  • GP1369 (''[gene|7A606B8E952AE8CA4F9A62008BA4B156725BB5B5|ugtP]''::''spc''), available in [SW|Jörg Stülke]'s lab
  • BKE21920 (Δ[gene|7A606B8E952AE8CA4F9A62008BA4B156725BB5B5|ugtP]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE21920 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CAAGTAAATTCACCTCAATG, downstream forward: _UP4_TAATGGCGTACTTGAGAGCA
  • BKK21920 (Δ[gene|7A606B8E952AE8CA4F9A62008BA4B156725BB5B5|ugtP]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK21920 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CAAGTAAATTCACCTCAATG, downstream forward: _UP4_TAATGGCGTACTTGAGAGCA

    • Expression vector

  • pGP2571, for expression in ''B. subtilis'' (based on [SW|pBQ200], available in [SW|Jörg Stülke]'s lab
  • pGP2600, for expression/ purification from ''E. coli'' with N-terminal Strep-tag, in [SW|pGP172], available in [SW|Jörg Stülke]'s lab

    • References

    Reviews

  • 19680248,17662935,22575476

    • Original Publications

  • 9244290,18820022,17662947,9720862,22362028,15640167,23935518,22931116,24097947,25069588,27684739,26833415